Investigation of the cyt gene in Bacillus thuringiensis and the biological activities of Bt isolates from the soil of China |
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| Institution: | 1. Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, P.O Box 32, Khartoum-North, Sudan;2. Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan;3. Faculty of Medicine, University of Khartoum, Qasr Street, 11111 Khartoum, Sudan;4. Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan;5. Division of Bioinformatics, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan;6. Utrecht Centre for Tick-borne Diseases (UCTD), FAO Reference Centre for Ticks and Tick-borne Diseases, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands;7. Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa;8. Division of Collaboration and Education, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan |
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| Abstract: | The presence of cyt genes was investigated in 80 type strains of Bacillus thuringiensis and 143 isolates obtained from soil samples of China by PCR amplification using two pairs of primers for the cyt1 and cyt2 genes. Three type strains of serotypes H11ac, H14 and H36, eight isolates belonging to H3, H14, H18 and H21, and one isolate of unknown serotype harbored cyt genes. We also tested the cytolytic activity for mammal cells, the hemolytic activity for sheep erythrocytes and insecticidal activity against mosquitoes of five isolates that contained cyt genes but did not belong to B. thuringiensis serovar israelensis. The protein profiles of the five isolates were different from those of the type strains of B. thuringiensis serovar israelensis, and among the five isolates, only Y-5 showed mosquitocidal activity against larvae of Culex quinquefasciatus. All five of the isolates exhibited hemolytic activity, but only three could cause the cell death of A549 cells. The cytopathological changes induced by NX-4 in some A549 cells were characterized with cell-ballooning. |
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