Antimicrobial peptide KSL-W promotes gingival fibroblast healing properties in vitro |
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| Institution: | 1. KU Leuven – University of Leuven, Department of Microbiology and Immunology, Laboratory of Molecular Bacteriology, Rega Institute for Medical Research, Herestraat 49, box 1037, B-3000 Leuven, Belgium;2. KU Leuven – University of Leuven, Department of Microbiology and Immunology, Laboratory of Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium;3. KU Leuven – University of Leuven, Department of Microbiology and Immunology, Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, Herestraat 49, box 1043, B-3000 Leuven, Belgium;4. KU Leuven – University of Leuven, University Hospitals Leuven, Laboratory Medicine, Herestraat 49, box 7003, B-3000 Leuven, Belgium;1. I3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal;2. INEB — Instituto de Engenharia Biomédica, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal;3. Requimte, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal;4. CIQUP, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal;5. ICBAS — Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal;6. IPATIMUP — Institute of Molecular Pathology and Immunology of the University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal;7. IBMC — Instituto de Biologia Celular e Molecular, Unidade de Produção e Purificação de Proteínas, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal;8. Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal |
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| Abstract: | We investigated the effect of synthetic antimicrobial decapeptide KSL-W (KKVVFWVKFK) on normal human gingival fibroblast growth, migration, collagen gel contraction, and α-smooth muscle actin protein expression. Results show that in addition to promoting fibroblast adhesion by increasing F-actin production, peptide KSL-W promoted cell growth by increasing the S and G2/M cell cycle phases, and enhanced the secretion of metalloproteinase (MMP)-1 and MMP-2 by upregulating MMP inhibitors, such as tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 in fibroblasts. An in vitro wound healing assay confirmed that peptide KSL-W promoted fibroblast migration and contraction of a collagen gel matrix. We also demonstrated a high expression of α-smooth muscle actin by gingival fibroblasts being exposed to KSL-W. This work shows that peptide KSL-W enhances gingival fibroblast growth, migration, and metalloproteinase secretion, and the expression of α-smooth muscle actin, thus promoting wound healing. |
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| Keywords: | Antimicrobial peptide KSL-W Gingival fibroblasts F-actin Cell migration Cell cycle α-SMA |
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