Purification and function of two analgesic and anti-inflammatory peptides from coelomic fluid of the earthworm,Eisenia foetida |
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| Institution: | 1. Shenzhen Yantian Maternity and Child Health Care Hospital, Shenzhen 518000, Guangdong, China;2. Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China;3. Ethic Drug Screening & Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicine Resource, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, Yunan, China;4. Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China;1. Medical School, Henan University of Science and Technology, Luoyang 471003, China;2. The Key Laboratory of Pharmacology and Medical Molecular Biology, Henan University of Science and Technology, Luoyang 471003, China;1. State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China;2. Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China;3. Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin, 301617, China;4. School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China;5. Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China;6. Mudanjiang YouBo Pharmaceutical Co. Ltd, Mudanjiang, 157000, China;1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China;2. School of Pharmacy, Second Military Medical University, Shanghai, 200433, China;3. Institute of Interdisciplinary Complex Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China;1. Department of Life Science, College of Natural Science, Daejin University, Pocheon, Gyeonggido 487-711, South Korea;2. Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-707, South Korea;3. Department of Biomedical Engineering, Jungwon University, Goesan, Chungcheongbukdo 367-700, South Korea;4. School of Life Sciences, KNU Creative Bioresearch Group (BK21 Plus Program), College of Natural Sciences, Kyungpook National University, Daehak-ro 80, Buk-gu, Daegu 702-701, South Korea;5. School of Life Sciences, Gwangju Institute of Science and Technology, Oryong-dong, Buk-gu, Gwangju 500-712, South Korea |
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| Abstract: | The potential application of anti-inflammatory and analgesic compounds in medication and therapeutic care have become of increasing interest. We purified and characterized two novel analgesic and anti-inflammatory peptides, VQ-5 and AQ-5, from the coelomic fluid of the earthworm (Eisenia foetida). Their primary structures were determined as VSSVQ and AMADQ, respectively. Both peptides, especially AQ-5, exhibited analgesic activity in mouse models of persistent neuropathic pain and inflammation. AQ-5 also inhibited tumor necrosis factor alpha and cyclooxygenase-2 production. The mitogen-activated protein kinase signaling pathway, which is involved in analgesic and anti-inflammatory functions, was inhibited by AQ-5. Thus, the analgesic and anti-inflammatory effects of these peptides, especially AQ-5, demonstrated their potential as candidates for the development of novel analgesic medicines. |
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| Keywords: | Coelomic fluid Peptide Analgesic Anti-inflammation |
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