Synthetic peptide antigens derived from long-chain alpha-neurotoxins: Immunogenicity effect against elapid venoms |
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| Institution: | 1. Department of studies in Biochemistry University of Mysore, Manasagangothri, Mysore 570006, India;2. Division of Analytical Psychopharmacology, New York State Psychiatric Institute, USA;3. Department of Psychiatry, Orangeburg, NY 10962, USA;4. Nathan Kline Institute for Psychiatric Research, Orangeburg, NY 10962, USA;5. Post Graduate Department of Biochemistry, JSS College, Ooty Road, Mysore 570025, India;1. National Laboratory for Development of Endangered Drug Resources in Northwest China, College of Life Sciences, Shaanxi Normal University, Xi''an, Shaanxi, 710062, China;2. Innovation Center for Qinba Regions Sustainable Development, College of Life Sciences, Shaanxi Normal University, Xi''an, Shaanxi, 710062, China;3. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China;4. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;5. Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan |
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| Abstract: | Three-finger toxins (3FTXs), especially α-neurotoxins, are the most poorly neutralized elapid snake toxins by current antivenoms. In this work, the conserved structural similarity and motif arrangements of long-chain α-neurotoxins led us to design peptides with consensus sequences. Eight long-chain α-neurotoxins (also known as Type II) were used to generate a consensus sequence from which two peptides were chemically synthesized, LCP1 and LCP2. Rabbit sera raised against them were able to generate partially-neutralizing antibodies, which delayed mice mortality in neutralization assays against Naja haje, Dendrospis polylepis and Ophiophagus hannah venoms. |
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| Keywords: | Elapid antivenom Long-chain α-neurotoxins Snakebites Three-finger toxins |
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