The effect of CART on pituitary hormones release from cultured pituitary cells harvested from fasted and fed ad libitum male rats |
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| Affiliation: | 1. Department of Clinical Neuroendocrinology, Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813 Warsaw, Poland;2. Department of Neurology, Second Faculty of Medicine, Medical University of Warsaw, Bielanski Hospital, Ceglowska 80, 01-809 Warsaw, Poland;1. Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China;2. Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China;3. Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China;1. Department of Human Biology and Movement Sciences, Maastricht University Medical Center+, 6229 ER Maastricht, the Netherlands;2. Laboratory of Microbiology, Wageningen University, 6703HB Wageningen, the Netherlands;3. RPU Immunobiology, University of Helsinki, 00014 Helsinki, Finland;1. Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, College of Basic Medicine, Hubei University of Medicine, Hubei, China;2. Department of Clinical Laboratory, Dongfeng Hospital, Hubei University of Medicine, Hubei, China;3. Department of Clinical Laboratory, Shiyan Occupational Disease Hospital, Hubei, China;4. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei, China;1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Department of Chemistry (Ministry of Education), Northwest University, Xi’an, 710127, Shaanxi Province, China;2. Department of College of Chemistry & Chemical Engineering, Yan’an University, 580 Holy Road, Yan’an,716000, Shaanxi Province, China;1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqing RD 18, Beijing, 100085, China;2. University of Chinese Academy of Sciences, Yuquan RD 19 A, Beijing, 100049, China;1. Institute of Rheumatology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel;2. Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel;3. Department of Medicine H, Soroka Medical Center, Ben Gurion University of the Negev, Beer Sheva, Israel;4. Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel |
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| Abstract: | Cocaine and Amphetamine-Regulated Transcript (CART) is widely expressed in the central nervous system and in several endocrine organs. CART is an important factor in the regulation of energy homeostasis. The aim of the study was to assess the role of CART in physiological response of pituitary cells in a course of starvation. The pituitary cells harvested from starved and fed ad libitum male rats were cultured for 48 h and treated with: 0.1 nM, 1 nM, 10 nM or 100 nM doses of CART. The medium was collected after 60 min and stored at −70 °C until samples were further assayed for: LH, FSH, PRL, GH, TSH and ACTH. We revealed that in cultures of pituitary cells collected from fasted rats the basal levels of the examined hormones were reduced. Incubation of pituitary cells of non-starved rats with any dose of CART reduced the concentration of LH and TSH, while the levels of the other hormones were decreased after administration only specific doses of CART. In cells of fasted rats no change in the concentration of gonadotrophins was observed. The PRL level was increased only in the 1 nM dose of CART, while the 10 nM and 100 nM CART doses markedly enhanced GH and TSH. Moreover, administration of 1 nM, 10 nM and 100 nM of CART to cultured cells of fasted rats resulted in a significant rise of the ACTH.Our results indicate that CART can directly affect the physiological release of PRL, ACTH, TSH and GH in pituitary cells of starved animals. Moreover, CART did not alter the LH and FSH suppression level, which is correlated with food deprivation. This data stays in contrast with the already proposed role of CART as an anorexigenic hypothalamic factor. |
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| Keywords: | CART Pituitary hormones Starvation |
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