Identification of a high-molecular-weight (> 400 000) protein in hyaline cartilage |
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Authors: | Rose Spitz Fife Kenneth D. Brandt |
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Affiliation: | Rheumatology Division, Indiana University School of Medicine, 541 Clinical Drive, Indianapolis, IN 46223 U.S.A. |
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Abstract: | A high-molecular-weight (> 400 000) non-collagenous protein has been identified in normal articular cartilage from several mammalian species and in bovine tracheal cartilage. This protein is reduced by 2-mercaptoethanol to subunits with a molecular weight of 116 000, which appear to constitute approx. 2–4% of the total protein detectable by the Lowry assay in 4 M guanidinium chloride extracts of normal bovine and canine articular cartilage. Antiserum to the 116 kDa subunit protein from bovine articular cartilage cross-reacts with the intact and subunit proteins from bovine trachea and from normal canine, porcine and human articular cartilage. This protein is not found in non-cartilagenous tissues, suggesting that it is a cartilage-specific protein. We conclude that the > 400 kDa protein and its subunit are ubiquitous and quantitatively significant proteins in hyaline cartilage. |
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Keywords: | Non-collagen protein Cartilage |
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