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Simultaneous Demonstration of Nonspecific Esterase and Chloroacetate Esterase in Human Blood Cells |
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| Authors: | Kwok-Wai Lam Chin-Yang Li Lung T Yam |
| Institution: | a Departments of Ophthalmology and Biochemistry, Austin, Texas b Departments of Laboratory Medicine and Surgical Pathology, Mayo Clinic, Rochester, Minnesota c Division of Hematology, Veterans Administration Medical Center, Louisville, Kentucky |
| Abstract: | A new cytochemical method is described for the simultaneous demonstration of nonspecific esterase in monocytes and chloracetate esterase in granulocytes. The procedure uses both alpha-naphthyl butyrate and naphthol AS-D chloroacetate as substrates and hexazotized pararosaniline as the coupler. The enzyme reaction products are highly chromogenic and their localization is precise. This method is potentially useful for the accurate diagnosis of the acute monocytic leukemias. Its advantages and limitations are also discussed. |
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