首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Chromosomal Location of 46 New RAPD Markers in Rye (Secale Cereale L.)
Authors:Cecilia González  Manuel V Camacho  César Benito
Institution:(1) Laboratory of Genetics, Universidad San Pablo, CEU, Boadilla del Monte, E-28660 Madrid, Spain;(2) Department of Genetics, Faculty of Biology, Complutense University, E-28040 Madrid, Spain
Abstract:The polymerase chain reaction (PCR) was used to locate RAPD markers using disomic wheat–rye addition lines in order to develop a set of molecular markers distributed on the seven rye chromosomes. We carried out RAPD amplifications on genomic DNA of wheat lsquoChinese Springrsquo (CS), rye lsquoImperialrsquo (I), the amphiploid wheat–rye and the seven disomic wheat–rye addition lines (1R–7R) using 140 different 10-mer oligonucleotides. Forty six new RAPD markers were located on the seven rye chromosomes and all the disomic wheat–rye addition lines were identified on the basis of their amplification patterns. The number of RAPD bands located on 1R, 2R, 3R, 4R, 5R, 6R and 7R chromosomes were 5, 8, 11, 8, 8, 10 and 6, respectively. The seven wheat–rye addition lines can be distinguished using only the following three 10-mer oligonucleotides: OPA16, OPF19 and GEN3-605, the other RAPD primers being useful for this purpose. The use of these RAPDs as a source of molecular markers that could be linked to interesting genes or other important agronomic traits is discussed.
Keywords:chromosomal location  gene mapping  RAPDs  rye  Secale cereale L  
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号