| 短短小芽孢杆菌启动子筛选载体的构建 |
| |
| 引用本文： | 彭清忠,陈军,陈玲,彭清静.短短小芽孢杆菌启动子筛选载体的构建[J].生命科学研究,2010,14(2):117-121. |
| |
| 作者姓名： | 彭清忠 陈军 陈玲 彭清静 |
| |
| 作者单位： | 吉首大学生物资源与环境科学学院,中国湖南,吉首,416000 |
| |
| 基金项目： | 湖南省教育厅青年基会资助项目，湖南省重点学科建设项目 |
| |
| 摘 要： | 为了筛选短短小芽孢杆菌强启动子元件,应用PCR技术从枯草杆菌168中分离出α-淀粉酶基因,用其作为报告基因与质粒pUB110和pKF3一起构建了启动子筛选载体pKB/A.将短短小芽孢杆菌细胞壁蛋白基因启动子引入该载体构建成重组质粒pKB/PA,电穿孔法转化短短小芽孢杆菌50后发现α-淀粉酶以活性形式分泌表达.结果表明短短小芽孢杆菌启动子筛选载体构建成功.
|
| 关 键 词： | 短短小芽孢杆菌 启动子筛选载体 α-淀粉酶基因 α-amylase gene |
Construction of Promoter-screening Vectors of Brevibacillus brevis |
| |
| PENG Qing-zhong,CHEN Jun,CHEN Ling,PENG Qing-jing.Construction of Promoter-screening Vectors of Brevibacillus brevis[J].Life Science Research,2010,14(2):117-121. |
| |
| Authors: | PENG Qing-zhong CHEN Jun CHEN Ling PENG Qing-jing |
| |
| Institution: | PENG Qing-zhong,CHEN Jun,CHEN Ling,PENG Qing-jing (College of Biology , Environmental Sciences,Jishou University,Jishou 416000,Hunan,China) |
| |
| Abstract: | To screen highly active promoters of Brevibacillus brevis, α-amylase gene as a reporter gene was isolated from Bacillus subtilis 168 by PCR, and used to construct a promoter-screening vector pKB/A with pUB110 and pKF3. The promoter of the cell wall protein gene from Br. Brevis 50 was ligated to pKB/A, producing recombinant plasmid pKB/PA. After the recombinant plasmid pKB/PA was introduced into Br. Brevis 50 by electroporation, soluble and biologically active α-amylase was secreted directly into the culture medium. The results showed that a promoter-screening vector was constructed successfully. |
| |
| Keywords: | Brevibacillus brevis promoter-screening vectors |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
