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Killing of Bacillus subtilis spores by a modified Fenton reagent containing CuCl2 and ascorbic acid
Authors:Shapiro Michael P  Setlow Barbara  Setlow Peter
Affiliation:Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
Abstract:Bacillus subtilis spores were killed by CuCl(2)-ascorbic acid, chloride ions were essential for killing of spores, and spores with defective coats were killed more rapidly. CuCl(2)-ascorbic acid did not damage spore DNA, and spores killed by this reagent initiated germination. However, spores killed by CuCl(2)-ascorbic acid may have damage to their inner membrane.
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