Glucose oxidase overproducing mutants of Penicillium variabile (P16) |
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| Authors: | Maurizio Petruccioli Paola Piccioni Federico Federici Mario Polsinelli |
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| Affiliation: | Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, University of Basilicata, Via N. Saura 85,1-85100 Potenza, Italy; Dipartimento di Agrobiologia e Agrochimica, University of Tuscia, Via S.C. de Lellis, 1-01100 Viterbo, Italy; Dipartimento di Biologia Animale e Genetica, University of Florence, Via Romana, 17,I-50125 Florence, Italy |
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| Abstract: | Abstract Conidia of Penicillium variabile P16 were subjected to mutagenesis and selection for glucose oxidase production on media containing o -dianisidine. Studies of the relationship between dose of UV irradiation and conidial survival and frequency of mutation showed that the best frequency of positive mutation (17%) was obtained in correspondence to a conidial survival of 52%. Out of 54 overproducing mutants tested in shaken flasks, M-80.10 showed the highest level of glucose oxidase activity (127% higher than the wild-type). M-80.10 mutant, transferred every 15 days to fresh medium and tested monthly for 8 months, appeared stable. The time course of growth and enzyme production by the mutant M-80.10 showed an increase of the glucose oxidase activity in the culture medium up to 19 U ml−1 after 96 h of fermentation. |
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| Keywords: | Penicillium variabile UV mutagenesis Glucose oxidase production |
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