Biogenesis of mitochondria 48 |
| |
Authors: | D. J. Groot Obbink T. W. Spithill R. J. Maxwell Anthony W. Linnane |
| |
Affiliation: | (1) Department of Biochemistry, Monash University, 3168 Clayton, Victoria, Australia;(2) Present address: Microbiology Department, Royal North Shore Hospital, St., 2065 Leonards, N.S.W., Australia |
| |
Abstract: | Summary Commercial preparations of mikamycin have been shown to act as both inhibitors of mitochondrial protein synthesis and respiration. These preparations are shown to consist of two major streptogramin components (mikamycin A and mikamycin B) and a number of minor components. The major streptogramin components which inhibit mitochondrial protein synthesis in vitro are without effect in vivo due to whole cell impermeability to these compounds.A minor antimycin A-like component is the active compound in mikamycin preparations which inhibits growth of yeast cells on ethanol. The site of this inhibition is at the level of respiratory Complex III.The mitochondrial [mik 1-r] mutation confers resistance to this minor growth inhibitory component and cross resistance to antimycin A. For clarity the designation mik 1 has therefore been renamed ana1 to denote the mitochondrial determinant conferring resistance to antimycin A. Genetic and physical mapping studies localise the ana1 determinant in the region of mitochondrial DNA specifying cytochrome b. It is proposed that the ana1 locus is part of a gene specifying a membrane component of Complex III. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|