Effect of fraction V powder from bovine serum on rat liver microsomal glutathione S-transferase activity |
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| Authors: | H Nishino |
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| Affiliation: | Department of Immunology, School of Medicine, University of Occupational and Environmental Health, Japan. |
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| Abstract: | When bovine serum fraction V powder, which contains 98-99% albumin, is incubated with rat liver microsomes, the glutathione S-transferase activity is stimulated 3-fold. The fraction V stimulates the transferase activity even in microsomes pretreated with N-ethylmaleimide. There is no such stimulating activity in protein fractions other than fraction V. The corresponding cytosolic enzyme activity is not changed by fraction V powder or by N-ethylmaleimide. Neither treatment with charcoal nor heating in boiling water for 5 min can remove the stimulating activity from fraction V. |
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