Condensation of ninhydrin with aldehydes and primary amines to yield highly fluorescent ternary products. II. Application to the detection and assay of peptides, amino acids, amines, and amino sugars |
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| Authors: | K Samejima W Dairman J Stone S Udenfriend |
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| Affiliation: | 1. Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand;2. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand;3. Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand;4. Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand;5. Department of Radiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand;6. Center for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Science, Khon Kaen University, Khon Kaen, 40002, Thailand;7. Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand |
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| Abstract: | A procedure for assay of amino acids, peptides, and other primary amine containing compounds has been developed based on their inter-action with ninhydrin and phenylacetaldehyde to yield highly fluorescent ternary products. The fluorescent ninhydrin procedure is 10 to 100 times more sensitive than the colorimetric ninhydrin procedure. The fluorometric reaction has been successfully automated for column chromatography of amino acids and peptides and has been used for detecting primary amine containing compounds on paper and thin-layer chromatograms. |
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