A role for scavenger receptor B-I in selective transfer of rhodamine-PE from liposomes to cells |
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Authors: | Yan X Poelstra K Scherphof G L Kamps J A A M |
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Institution: | Department of Cell Biology, Groningen University Institute for Drug Exploration, University of Groningen, The Netherlands. |
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Abstract: | We investigated the potential role of scavenger receptor B-I (SR-BI) in the selective removal of liposomal markers from blood by hepatocytes. Liposomes were labeled with (3)H]cholesteryloleyl-ether ((3)H]COE), 1,2-di1-(14)C]palmitoyl-phosphatidylcholine ((14)C]PC), and N-(lissamine rhodamine-B sulfonyl)-phosphatidylethanolamine (N-Rh-PE). The radiolabels were eliminated at identical rates from plasma, while N-Rh-PE was cleared twice as fast. Involvement of SR-BI in the selective removal of N-Rh-PE from liposomes was studied in transfected Chinese hamster ovary cells over-expressing SR-BI. Uptake of N-Rh-PE from liposomes containing phosphatidylserine was higher than (3)H]COE, and was further enhanced by apolipoprotein A-I, confirming involvement of SR-BI in the selective uptake of liposomal N-Rh-PE by cells. |
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Keywords: | Liposomes Pharmacokinetics Hepatocytes Selective lipid transfer Scavenger receptor class B type I Apolipoprotein A-I |
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