Extension of anther culture to several genotypes of cultivated oats |
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Authors: | E Kiviharju M Puolimatka M Saastamoinen E Pehu |
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Institution: | (1) Agricultural Research Centre of Finland, Plant Production Research, Crops and Soil, FIN-31600 Jokioinen, Finland e-mail: elina.kiviharju@mtt.fi Fax: +358-3-41882496, FI;(2) Boreal Plant Breeding, FIN-31600 Jokioinen, Finland, FI;(3) Department of Plant Production, P.O. Box 27, FIN-00014 University of Helsinki, Finland, FI |
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Abstract: | To improve plant regeneration from oat anther culture, the basic medium, hormonal supplements and genotype effect were studied.
Six of the 14 genotypes tested regenerated plants. Cultivars Kolbu, Katri, Stout and naked oat Lisbeth produced green plants,
cultivars Virma and line OT 257 only albinos. The total number of green plantlets regenerated was 22, of which 13 (11 haploid,
2 doubled haploid) survived into the greenhouse, and 37 albinos. Regenerable-type embryos were induced from heat-pretreated
anthers on media containing 2, 3 or 5 mg l–1 2,4-dichlorophenoxyacetic acid and 0.2 or 0.5 mg l–1 kinetin as hormonal supplements. 6-Benzylaminopurine promoted albino plant regeneration especially in W14 medium. Colchicine treatment was applied successfully to haploid regenerants.
Received: 12 April 1999 / Revision received: 19 August 1999 / Accepted: 8 September 1999 |
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Keywords: | Androgenesis Avena sativa Chromosome doubling Haploids Naked oat |
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