| mirRNA-17-5p抑制物对骨肉瘤细胞系SOSP_9607增殖和凋亡的影响 |
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| 引用本文： | 赵广义,赵健,高杰,许啸,闫康,吴家昌,杨彤涛,马保安.mirRNA-17-5p抑制物对骨肉瘤细胞系SOSP_9607增殖和凋亡的影响[J].现代生物医学进展,2010,10(8). |
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| 作者姓名： | 赵广义 赵健 高杰 许啸 闫康 吴家昌 杨彤涛 马保安 |
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| 作者单位： | 第四军医大学唐都医院全军骨科中心暨全军骨肿瘤研究所; |
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| 摘 要： | 目的:探讨miR-17-5p抑制物对于骨肉瘤细胞系SOSP_9607细胞增殖和凋亡的影响.方法:四甲基偶氮唑蓝(MTT)法测定细胞增殖,进一步计算抑制率,流式细胞仪测定细胞凋亡.将SOSP_9607细胞分为对照组和实验组,对照组分为阴性对照和正常细胞对照组.实验组采用miR-17-5p抑制物(hsa-miR-17-5p inhibitors)抑制SOSP_9607细胞内miR-17-5p的活性.结果:与对照组相比,实验组显著抑制SOSP_9607细胞的增殖,有明显的剂量依赖性(P<0.01).随着浓度从50 nmol/L逐渐增加至200 nmol/L,抑制率逐渐增高(P<0.01).实验组凋亡率(9.6±1.8)%与阴性对照组凋亡率(3.5±0.4)%相比明显增高(P<0.01).结论:miR-17-5p抑制物通过抑制SOSP_9607细胞中miR-17-5p的活性对SOSP_9607细胞的增殖和凋亡发挥重要作用.
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| 关 键 词： | 骨肉瘤 成骨肉瘤细胞系SOSP_9607 miR-17-5p 细胞增殖 细胞凋亡 |
Effects of MicroRNA-17-5p inhibitors on Proliferation and Apoptosis of Human Osteosarcoma Cell Line SOSP-9607 |
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| ZHAO Guang-yi,ZHAO Jian,GAO Jie,XU Xiao,YAN Kang,WU Jia-chang,YANG Tong-tao,MA Bao-an.Effects of MicroRNA-17-5p inhibitors on Proliferation and Apoptosis of Human Osteosarcoma Cell Line SOSP-9607[J].Progress in Modern Biomedicine,2010,10(8). |
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| Authors: | ZHAO Guang-yi ZHAO Jian GAO Jie XU Xiao YAN Kang WU Jia-chang YANG Tong-tao MA Bao-an |
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| Institution: | ZHAO Guang-yi,ZHAO Jian,GAO Jie,XU Xiao,YAN Kang,WU Jia-chang,YANG Tong-tao,MA Bao-an (Department of Orthopedic Surgery Center and Orthopedic Oncology Institute of PLA,Tangdu Hospital,Fourth Military Medical University,Xi,an,Shaanxi,710038,P.R.China) |
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| Abstract: | Objective:To determine the effects of microRNA-17-5p inhibitors on proliferation and apoptosis of human osteosarcoma cell line SOSP-9607.Methods:MTT assay was used to examine the inhibitive percentage of hsa-miR-17-5p inhibitors on the proliferation of SOSP-9607 cells.Cultured SOSP-9607 cells were divided into two groups:control group and experimental group.The control group contained two groups:the negtive control and the normal control.In experimental group,hsa-miR-17-5p inhibitors were used to inhibit th... |
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| Keywords: | osteosarcoma SOSP-9607 cell line miR-17-5p cell proliferation cell apoptosis |
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