DNA hybridization sensor based on pentacene thin film transistor |
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Authors: | Kim Jung-Min Jha Sandeep Kumar Chand Rohit Lee Dong-Hoon Kim Yong-Sang |
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Institution: | Department of Nano Science & Engineering, Myongji University, Gyeonggi-do 449-728, Republic of Korea. |
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Abstract: | A DNA hybridization sensor using pentacene thin film transistors (TFTs) is an excellent candidate for disposable sensor applications due to their low-cost fabrication process and fast detection. We fabricated pentacene TFTs on glass substrate for the sensing of DNA hybridization. The ss-DNA (polyA/polyT) or ds-DNA (polyA/polyT hybrid) were immobilized directly on the surface of the pentacene, producing a dramatic change in the electrical properties of the devices. The electrical characteristics of devices were studied as a function of DNA immobilization, single-stranded vs. double-stranded DNA, DNA length and concentration. The TFT device was further tested for detection of λ-phage genomic DNA using probe hybridization. Based on these results, we propose that a "label-free" detection technique for DNA hybridization is possible through direct measurement of electrical properties of DNA-immobilized pentacene TFTs. |
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