| C群脑膜炎球菌多糖纯化方法的改进 |
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| 引用本文： | 杨明,陈作江,曹鑫,王志军,崔萱林.C群脑膜炎球菌多糖纯化方法的改进[J].微生物学免疫学进展,2006,34(4):27-29. |
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| 作者姓名： | 杨明 陈作江 曹鑫 王志军 崔萱林 |
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| 作者单位： | 兰州生物制品研究所,兰州,730046 |
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| 摘 要： | 将C群脑膜炎球菌粗多糖的纯化改进为用1:1容量冷酚提取的生产工艺。结果表明,改进后的方法去除蛋白质效果同样能够达到《中国药典》2005版(三部)的要求,总体结果优于改进前。同时能扩大处理量而降低了生产成本,适合规模化生产。
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| 关 键 词： | C群脑膜炎球菌多糖 苯酚 抽提 蛋白质 |
| 文章编号： | 1005-5673（2006）04-0027-03 |
| 收稿时间： | 2006-06-08 |
| 修稿时间： | 2006-07-12 |
Improvement of purification of polysaccharide from group C meningococcus |
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| YANG Ming , CHEN Zuo-jiang , CA Xin ,et al..Improvement of purification of polysaccharide from group C meningococcus[J].Progress In Microbiology and Immunology,2006,34(4):27-29. |
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| Authors: | YANG Ming CHEN Zuo-jiang CA Xin |
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| Institution: | Lanzhou Institute of Biological Products ,Lanzhou 730046, China |
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| Abstract: | The purification of polysaccharide from capsule of group C meningococcus was improved to extract crude polysaccharide with a single volume of cold phenol.On the basis of the improved method,the residual protein is still below the quality control standard in "Pharmacopoeia of the People's Republic of China "2005 edition,furthermore,the quality of purified polysaccharide is even better than that prepared by using the previous method.This new method could be used in a large-scale purification with a lower cost in production. |
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| Keywords: | Group C meningococcal polysaccharide Phenol Extraction Protein |
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