A Precise Technique for Measurement of Roots and Root Distribution in Soils

A procedure is described in detail which yields directly thecomplete spatial distribution and sizes of roots in soils, andso provides such information as the length and surface areaof root per unit volume, the number of root tips per unit volume,and the proximity of roots to one another. The method involves stabilization of the soil by vacuum impregnationwith Plastrene 47 and examination of the serial surfaces preparedby surface-grinding, using both visible and ultraviolet fluorescencephotography.