Detection and Incidence of Specific Species of Spoilage Bacteria on Fish: I. Methodology |
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Authors: | R. E. Levin |
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Abstract: | The ability of Pseudomonas putrefaciens to form H(2)S was found to serve as a singularly useful criterion of identity for this species and was used to directly enumerate the organism from haddock fillets by the use of pour plates of Peptone-Iron Agar. Subsurface colonies appear intensely black, whereas surface colonies are black or gray. A highly sensitive soft-agar-gelatin overlay technique has been found useful for directly determining the numbers of weakly and strongly proteolytic organisms from fish tissue. |
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