Methodology of reversible protein labeling for ratiometric fluorescent measurement |
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| Authors: | Soh Nobuaki Seto Daisuke Nakano Koji Imato Toshihiko |
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| Affiliation: | Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan. |
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| Abstract: | The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique amino acid tag. |
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