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A synergistic effect of glutathione-depletion and elicitation on the production of 6-methoxymellein in carrot cells |
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| Authors: | Ze-jian Guo Yoshimoto Ohta |
| Affiliation: | (1) Yamazaki Laboratory, PCC Technology Inc., Suntory Research Center, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, 618 Osaka, Japan;(2) Present address: Supermolecules Project (JRDC), Keihanna Plaza, Seika-cho 1-7, Soraku-gun, 619-02 Kyoto, Japan;(3) Department of Biotechnological Science, Kinki University, Uchita-cho, 649-64 Wakayamaken, Japan |
| Abstract: | The effects of buthionine sulfoximine (BSO) and yeast glucan elicitor (YE) on the production of 6-methoxymellein (6-MM) and generation of H2O2 in suspension-cultured carrot cells were examined. Administration of BSO and YE together affected the cells synergistically to lead to an enhanced production of 6-MM. These data indicate the significance of formation and decay of active oxygen species as a second signal of elicitation in triggering the biosynthesis of the phytoalexin.Abbreviations BSO buthionine sulfoximine - MDA malondialdehyde - 6-MM 6-methoxymellein - YE yeast glucan elicitor |
| Keywords: | Daucus carota active oxygen species elicitor phytoalexin |
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