Effective plasmid pX3 transduction in Lactobacillus delbrueckii by bacteriophage LL-H |
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Authors: | Ravin Victor Sasaki Takashi Räisänen Liisa Riipinen Katja-Anneli Alatossava Tapani |
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Affiliation: | aDepartment of Biology, University of Oulu, Linnanmaa campus, P.O. Box 3000, FIN-90014 Oulu, Finland;bFood Science Institute, Meiji Dairies Corporation, Odawava, Kanagawa 250-0862, Japan;cDepartment of Food Technology, University of Helsinki, Viikki campus, P.O. Box 66, FIN-00014 Helsinki, Finland |
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Abstract: | High-frequency plasmid transductions in Lactobacillus delbrueckii subsp. lactis and subsp. bulgaricus strains mediated by pac-type bacteriophages were observed and further investigated. The frequency of plasmid transduction by phages LL-H and LL-S attained levels of from 0.10 to about 1 with plasmid pX3, but only about 2 × 10−2 with plasmid pJK650. Infection of L. delbrueckii subsp. lactis strain LKT(pX3) or ATCC 15808(pX3) with phage LL-H resulted in intensive concatemerization of plasmid pX3, and most progeny phage particles contained concatemers of plasmid DNA instead of phage LL-H DNA. The synthesis of phage LL-H DNA was depressed. No evident homology or recombination was observed between phage LL-H DNA and plasmid pX3. The unusually high frequency of plasmid pX3 transduction by phage LL-H could be considered to result from specific interaction(s) between a particular phage and plasmid. These interactions may include pX3-mediated blockage of phage LL-H DNA replication and effective use of a particular pac-like site located about 1 kb from BglII in the smaller NdeI-BglII fragment of plasmid pX3. Phage LL-H together with plasmid vector pX3 could be used as effective plasmid transduction tools for genetic engineering of L. delbrueckii subsp. lactis and subsp. bulgaricus strains. |
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Keywords: | Lactic acid bacteria Lactobacillus delbrueckii Bacteriophages Transduction Plasmid, Genetic engineering |
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