Chitosomes lack concanavalin-A-binding sites |
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Authors: | Prof Salomon Bartnicki-Garcia Ildiko Bartnicki Rafael Sentandreu |
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Institution: | (1) Department of Plant Pathology, University of California, 92521 Riverside, California, USA;(2) Departamento de Microbiología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain |
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Abstract: | Whether intact or dissociated with digitonin, chitosomes isolated from the fungusMucor rouxii lack the ability to bind concanavalin A. The absence of external or internal concanavalin A-binding sites distinguishes the chitosome membrane no only from plasma membrane but also from membranes of other organelles (endoplasmic reticulum, mitochondrion, vacuole). This differential binding ability was used to partially separate chitosomal chitin synthetase from major membranes in a crude cell-free extract ofM. rouxii. |
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