表面活性剂对绿色木霉产纤维素酶影响

利用绿色木霉，以稻草为唯一碳源，采用液态发酵的方法，分别加入生物表面活性剂鼠李糖脂和化学表面活性剂Tween 80，重点研究了生物表面活性剂对绿色木霉产纤维素酶的影响。实验分析了加入不同浓度的表面活性剂时滤纸酶活、羧甲基纤维素酶活、微晶纤维素酶活及酶液的表面张力随时间的变化情况。结果表明，添加鼠李糖脂能够促进绿色木霉产酶，分别使滤纸酶活、羧甲基纤维素酶活、微晶纤维素酶活最大提高了1.08倍，1.6倍和1.03倍。与Tween 80相比，鼠李糖脂促进产酶的效果明显优于Tween 80。

Experimental Study on the Effects of surfactants on Cellulase from Trichoderma viride

The effects of surfactants on the production of cellulase by Trichoderma viride in liquid substrate fermentation process were investigated. Straw was used as the sole carbon source and the surfactants were biosurfactant rhamnolipid from Pseudomonas aeruginosa and Tween 80. The changes of FPA,CMCase,Avicelase and surface tension with time were analyzed under different concentrations of the two surfactants. The results showed that the surfactants can enhance the enzyme activity of Trichoderma viride. The FPA,CMCase,Avicelase were promoted 1.08,1.6 and 1.03 times higher than the controls by rhamnolipid. The enhancement of the enzyme activity by rhamnolipid was much higher than that of Tween 80. At the same time,rhamnolipid was not degraded prior to other substrate.