Regeneration of garlic plants (allium sativum L., CV. “Chonan”) via cell culture in liquid medium |
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摘 要: | Summary Aiming at the genetic improvement of garlic cultivars, a cell suspension protocol was established which includes the induction
of friable callus, establishment of cells in liquid medium, plating, regeneration, and bulb formation. Calluses of various
textures from compact to friable and from green to yellowish were obtained by culturing explants excised from inner leaves
of garlic bulbs on Marashig-Shoog (MS) medium with 2,4 dichlorophenoxy acetic acid (2,4-D), (1.1 mg/liter 5.0 μM]), picloram
(1.2 mg/liter 5.0 μM]), and kinetin (2.1 mg/liter 10 μM]). Friable callus occurred on MS-A contained 2,4-D alone (1.0 mg/liter
4.52 μM]) and this callus was used to develop cell suspension cultures, which were maintained in liquid MS-B medium with a 2,4-D/benzyl
adenine (BA) (0.5 mg/liter 2.25 μM]: 0.5 mg/liter 2.22 μM]) ratio. High plating efficiency was obtained on MS-C medium with different naphthalene acetic acid/BA combinations. Regeneration
occurred after transfer of the caulogenic mass to MS-C medium containing 10 mg/liter (74.02 μM) and 20 mg/liter (148.04 μM) adenine for 60 days, followed by transfer to adenine-free medium. Plantlets transplanted to soil showed normal phenology.
Shoots grown on modified MS medium supplemented with indolylbutryic acid (3.0 mg/liter 14.7 μM]) stimulated bulb formation by 30 days in culture.
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收稿时间: | 16 July 1993 |
修稿时间: | 7 April 1994 |
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