| 黄瓜Ri T—DNA转化根的植株再生 |
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| 引用本文： | 刘伟华,张艳馥. 黄瓜Ri T—DNA转化根的植株再生[J]. 生物技术, 1998, 8(4): 23-26 |
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| 作者姓名： | 刘伟华 张艳馥 |
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| 作者单位： | [1]哈尔滨师范大学生物系 [2]克山师专生物系 |
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| 基金项目： | 蔡火石生物科学发展基金 |
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| 摘 要： | 本文利用发根农杆菌感染黄瓜植体得到的转化根，在不同激素配比的分分培养基上培养，进行再生植株的诱导试验，结果表明，ＮＡＡ和ＢＡ的合适配比，有利于黄瓜植株的再生，转化根及其再生植株检测到冠瘿碱的存在，说明农杆菌Ｒｉ质粒的Ｔ－ＤＮＡ已转移到黄瓜基因组中。
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| 关 键 词： | 黄瓜 T－DNA 转化 再生 |
PLANT REGENERATION OF Ri T-DNA TRANSFORMED ROOTS OF CUCUMBER |
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| Liu Weihua, Shi Rui, Xu Xianging, Li Jilin. PLANT REGENERATION OF Ri T-DNA TRANSFORMED ROOTS OF CUCUMBER[J]. Biotechnology, 1998, 8(4): 23-26 |
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| Authors: | Liu Weihua Shi Rui Xu Xianging Li Jilin |
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| Affiliation: | Liu Weihua, Shi Rui, Xu Xiang1ing, Li Jilin |
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| Abstract: | Transgenic roots were induced by affection of A g roba ctertu m rhizog enes with Ri p1asmicon cucumber exp1ants. Severa1 culture medium with varying hormone were screened fc-regeneration. res u1ts shov' the appropriate concentration of naphthaleneacetu (N A A ) and 6- benzy1aden1ne (BA ) u'as 1mportant for regeneration process. The transformed nat urc o1tlle roots anQ oi the regenerating plants was comfirmed by the presence of opine' |
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| Keywords: | cucumber T-DNA transformation regeneration |
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