Replication timing properties across the pseudoautosomal region boundary and cytogenetic band boundaries on human distal Xp |
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Authors: | Kristin Bilyeu A Craig Chinault |
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Institution: | (1) Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA, US;(2) Program in Cell and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA, US;(3) Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA, US |
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Abstract: | The establishment of human chromosomal regions as distinct and characteristic domains has been demonstrated by the reproducible
banding patterns observed on metaphase chromosomes as a result of various staining techniques. Although the exact molecular
properties responsible for the patterns are not well understood, a general correlation has been established between the time
of replication of a particular region of DNA and its banding characteristics. Using a replication timing assay based on fluorescence
in situ hybridization patterns, we investigated replication timing properties across chromosomal regions with potentially
distinct chromatin properties. Relative replication timing values were determined using cosmid DNA probes around the pseudoautosomal
region boundary in Xp22.3 and the cytogenetic band boundary regions surrounding Xp22.2. Although we observed replication timing
domains that were generally consistent with cytogenetic banding patterns, we did not find sharp replication timing boundaries
at either the pseudoautosomal region boundary or at the cytogenetic band boundaries.
Received: 6 September 1997; in revised form: 16 December 1997 / Accepted: 5 January 1998 |
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