Fine specificity of cytotoxic T lymphocytes directed againstH-2L
d |
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Authors: | C J M Melief L P de Waal M Y van der Meulen P Iványi R W Melvold |
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Institution: | (1) Department of Tumor Immunology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands;(2) Department of Immunohematology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands;(3) Laboratory for Experimental and Clinical Immunology, University of Amsterdam, Amsterdam, The Netherlands;(4) Section of Medical Oncology, Departments of Medicine and Microbiology-Immunology, Northwestern University, 303 E. Chigaco Avenue, 60611 Chicago, Illinois |
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Abstract: | The fine specificity of cytotoxic T lymphocytes (CTL) directed againstH-2L
d
was analyzed by studying the lytic activity of BALB/cH- 2dm2 (H-2L
d
loss mutant) anti-BALB/c-H-2
d
CTL, generated in secondary mixed lymphocyte culture (MLC) against a panel of target cells of differentH-2 haplotypes. Target cells of allH-2 haplotypes tested, except that of the MLC responder, were lysed by anti-Ld CTL, although to a widely varying extent. The genes coding for antigens detected by anti-L
d
CTL were mapped to distinct regions in theH-2
d
,H- 2dm1,H-2
q
,H-2
k
, andH-2
b
haplotypes. The sequence of lysis intensity against the variousH-2 haplotypes and theH-2 regions involved were as follows:L
d
,D
q
L
q
,D
dm1 Ldm1,K
k
,D
b
L
b
,r, p, f, s, C3H.OH (K
d
D
k
L
k
), strong lysis occurring againstL
d
and weak lysis againstH-2
s
and C3H.OH.By monolayer adsorption and cold target inhibition experiments, it was shown that anti-L
d
CTL contained a CTL subset directed against a private Ld specificity, hitherto undetected by anti-L
d
antibodies. This subset of CTL was separate from the CTL subsets reacting againstH-2
q
and against the mutant haplotypeH- 2dm1. The reactions against the latter two haplotypes were also mediated by separate CTL subsets. It is concluded that the Ld molecule, to a varying extent, shares target antigens for CTL with K- and/or D-end H-2 molecules of all haplotypes tested. These antigens are detected by multiple subsets of anti-L
d
CTL. One CTL subset is directed against a target structure unique forL
d
(Ld private specificity). |
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Keywords: | |
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