Elucidation of the interactions of an anticancer ruthenium complex in clinical trials with biomolecules utilizing capillary electrophoresis hyphenated to inductively coupled plasma-mass spectrometry. Short communication |
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Authors: | Groessl Michael Hartinger Christian G Połeć-Pawlak Kasia Jarosz Maciej Dyson Paul J Keppler Bernhard K |
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Affiliation: | University of Vienna, Institute of Inorganic Chemistry, Waehringer Strasse 42, A-1090 Vienna. |
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Abstract: | The application of capillary electrophoresis (CE) combined with highly sensitive inductively-coupled-plasma mass spectrometric (ICP-MS) detection allows the interactions of metal complexes with biomolecules to be characterized. This technique has been used to provide new insights into the mode of action of the ruthenium-based anticancer drug candidate indazolium [trans-tetrachlorobis(1H-indazole)ruthenate(III)] (KP1019). While the compound binds rapidly and efficiently to serum proteins, especially albumin, its reactivity towards the model DNA compound 2'-deoxyguanosine 5'-monophosphate (5'-dGMP) is moderate. |
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Keywords: | Anticancer research Capillary electrophoresis (CE) Mass spectrometry KP1019 DNA Ruthenium complexes |
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