Effects of brefeldin A on the synthesis of chondroitin 4-sulfate by cultures of mouse mastocytoma cells. |
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Authors: | G Sugumaran M Katsman J E Silbert |
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Institution: | Connective Tissue Laboratory, Department of Veterans Affairs Outpatient Clinic, Boston, MA 01730. |
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Abstract: | Mouse mastocytoma cells were cultured with brefeldin A in medium containing 35S]sulfate and 3H]glucosamine in order to determine the effects of this fungal metabolite on the formation of chondroitin 4-sulfate by these cells. There was a marked reduction in the incorporation of 35S]sulfate into the glycosaminoglycan which was approximately equal to the reduction in the incorporation of 3H]hexosamine into the same molecule. The chondroitin 4-sulfate chain size was greatly diminished, while the number of chains appeared to remain relatively constant, indicating that the brefeldin A partially disrupted the polymerizing system, but had little effect upon movement of the nascent proteochondroitin to the site for chondroitin polymerization and sulfation. |
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