Detection of glycoproteins in Borrelia burgdorferi |
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| Authors: | Vittorio Sambri Claudio Stefanelli Roberto Cevenini |
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| Institution: | (1) Institute of Microbiology, University of Bologna, S. Orsola Hospital-9, via Massarenti, I-40138 Bologna, Italy;(2) Department of Biochemistry, University of Bologna-48, via Irnerio, I-40138 Bologna, Italy |
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| Abstract: | The presence of carbohydrates on proteins of Borrelia burgdorferi, the causative agent of Lyme disease, was investigated by using a digoxigenin labeling method together with Schiff staining and N-glycosidase F assay. The two major outer surface exposed proteins of 31 kDa and 34 kDa showed to be glycosylated and gel filtration high pressure liquid chromatography (HPLC) of proteins of B. burgdorferi metabolically labeled with 14C-N-acetylglucosamine revealed the incorporation of the carbohydrate into the glycosyl residue of these proteins.Abbreviations
N-glycosidase F
peptide-N-glycosidase F (EC 3.5.1.52)
- SDS-PAGE
sodium dodecyl sulphate polyacrylamide gel electrophoresis
- WB
Western blotting
- HPLC
high pressure liquid chromatography
- SDS
sodium dodecyl sulphate
- mAb
monoclonal antibody
- MIAF
mouse immune ascitic fluid
- SS
Schiff staining
- Osp
Outer surface protein |
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| Keywords: | Borrelia burgdorferi Glycoproteins Digoxigenin labeling Monoclonal antibodies N-Glycosidase F Gel filtration HPLC |
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