DETECTION AND ENUMERATION OF H2S+ BACTERIA: APPLICATION TO SHEWANELLA PUTREFACIENS (CIP) |
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| Authors: | P. MALLE,M. VALLÉ ,P. DEMARQUE,P. EB,R. TAILLIEZ |
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| Affiliation: | CNEVA (Centre National d'Etudes Vétérinaires et Alimentaires) Laboratoire d'études des produits de la pêche rue Huret Lagache 62200 Boulogne sur mer, France;IAAL (Institut Agricole et Alimentaire de Lille) Laboratoire de microbiologie 59655 Villeneuve d'Ascq Cedex, France |
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| Abstract: | H2S+ bacteria responsible for the degradation of sulfur-containing amino acids of fish muscle are currently little used to evaluate the microbiological pal quality of fish. Shewanella putrefaciens greatly predominates in this flora, and was therefore used to define a suitable culture method and medium. Inoculations by the Spiral surface method at 25C, with an incubation of 72h, gave the best counts on a medium containing two sources of sulfur (organic and inorganic) for H2S+ bacteria. The culture medium and the NaCl concentration were determinant in the evaluation of this flora. At present there is no standard medium which meets these requirements. |
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