Role of a microbial factor, necrotic tissue and a foreign body in the development of wound suppuration

The effect of microorganisms, necrotic tissues and a foreign body on the development of festering process in rat wounds was studied. It was found that the presence of necrotic tissues is the necessary and sufficient condition for a clinical manifestation of infection in rat wounds. Additional infection of the wounds and introduction of a foreign body did not appreciably change the clinical picture. A model is suggested of a festering wound in rats without artificial infecting. In the festering wound, one could observe the replacement of the gram-positive coccal microflora by the gram-negative one. Meanwhile the gram-positive coccal microflora was predominant in the non-festering wound. The level of potential biochemical indicators of infection in the blood of animals with festering wounds was higher than in the blood of those with non-festering wounds. The morphology, and the content of microbial cells, the content of nucleic acids, and total proteolytic activity in festering wound tissues were examined over time.