YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality |
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Authors: | Yodmuang Supansa Tirasophon Witoon Roshorm Yaowaluck Chinnirunvong Wanlop Panyim Sakol |
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Institution: | The Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Nakornpathom 73170, Thailand. |
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Abstract: | Yellow head virus infects cultured shrimps and causes severe mortality resulting in a great economic loss. Haemolymph injection of dsRNA(pro) corresponding to the protease motif of YHV genome resulted in a complete inhibition of YHV replication. The effect of dsRNA lasted for at least 5 days. Injecting sequence-unrelated dsRNA(gfp) or dsRNA(TSV-pol) also resulted in an inhibition of YHV replication but at a comparatively much less extent. Shrimp mortality was monitored for 10 days when more than 90% shrimps receiving no dsRNA died within 8 dpi. However, those receiving dsRNA(pro) showed no mortality. A partial mortality was observed among the shrimps receiving dsRNA(gfp) or dsRNA(TSV-pol). Thus, Penaeus monodon possesses the sequence-specific protection to YHV infection, most likely through the RNAi pathway, in addition to sequence-independent protection. It gives a new notion that dsRNA induction of antiviral immunity in shrimp goes through two pathways, sequence-independent and sequence-dependent. |
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Keywords: | RNA interference Double-stranded RNA Yellow head virus Penaeus monodon |
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