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Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum |
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| Authors: | Duong Tan Nhut Bui Van Le Nguyen Tri Minh Jaime Teixeira de Silva Seiichi Fukai Michio Tanaka K Tran Thanh Van |
| Institution: | (1) Institute of Biology in Dalat, 116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam (;(2) College of Natural Science, Vietnam National University-Ho Chi Minh City, Department of Biotechnology, Faculty of Biology, 227 Nguyen Van Cu, Distrist 5, Ho Chi Minh City, Vietnam;(3) Departamento de Biologia Vegetal, Faculdade de Ciências de Lisboa, C-2 Campo Grande, 1780 Lisboa, Portugal;(4) Faculty of Agriculture, Kagawa University, 761-0795 Miki-cho, Japan;(5) Institut de Biotechnologie des Plantes, UMR 0569, Universitê de Paris-Sud, F-91405 Orsay Cedex, France |
| Abstract: | Somatic embryogenesis was achieved directly from pseudo-bulblettransverse thin cell layers (tTCL) of Lilium longiflorum.Embryo-like structures (globular embryos) were obtained from different sizeexplants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog,1962 (MS) medium containing 5.4  M naphthalene acetic acid(NAA)and 1.1 M thidiazuron (TDZ). The embryo-like structures werethen isolated and mass proliferated on MS medium, containing 5.4M NAA and 0.4 M TDZ, every 45 days. A0.8–1.0 mm thick explant was shown to be optimal forobtaining the highest number of embryo-like structures. For plant regenerationthese structures were transferred to hormone-free MS medium with 30g/l sucrose. All of these structures formedplantlets after 90 days culture. |
| Keywords: | Globular embryo Lilium longiflorum Pseudo-bulblet Regeneration Sucrose Thin cell layer |
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