Non-toluene-associated respiration in a Pseudomonas putida 54G biofilm grown on toluene in a flat-plate vapor-phase bioreactor |
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Authors: | S Villaverde M T Fernández |
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Institution: | (1) Departament d'Enginyeria Química, Escola Tècnica Superior d'Enginyeria Química, Universitat Rovira i Virgili, Carretera de Salou s/n, Tarragona E-43006, Spain Tel.: 34 77 559671 Fax: 34 77 559621, ES |
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Abstract: | Non-toluene-associated respiration (NTAR) within a Pseudomonas putida 54G biofilm growing on toluene as sole external carbon source was evaluated using oxygen microelectrodes in a flat-plate
vapor-phase biological reactor. Two fluorescent probes, 2,4-diamidino-2-phenylindole and 5-cyano-2,3-ditolyltetrazolium chloride,
were used to evaluate the number of total and respiring cells respectively within the biofilm. Biofilm samples were also analyzed
for viable and toluene-culturable cells by spread-plating on non-selective and selective media respectively. Fractions of
viable stressed, respiring and non-respiring cells within the biofilm were evaluated. The NTAR rate was positively correlated
with the fraction of viable stressed and non-respiring cells within the biofilm, which suggested the capability of some cells
to grow at the expense of leakage and lysis products coming from injured and dead cells. This effect was more pronounced at
higher toluene concentration. Results suggest that NTAR should be incorporated into mathematical models of biofilm reactors
degrading volatile organic carbon compounds.
Received: 4 January 1997 / Received revision: 20 March 1997 / Accepted: 27 March 1997 |
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