The unsaturation of phosphatidylglycerol in thylakoid membrane alleviates PSII photoinhibition under chilling stress |
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Authors: | Xiao-lin Sun Sha Yang Li-Yan Wang Qiu-Yu Zhang Shi-Jie Zhao Qing-Wei Meng |
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Institution: | (1) College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018, People’s Republic of China; |
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Abstract: | Over-expression of chloroplast glycerol-3-phosphate acyltransferase gene (LeGPAT) in tomato increased cis-unsaturated fatty acid content in phosphatidylglycerol (PG) of the thylakoid membrane. Under chilling stress, the oxygen
evolving activity, the maximal photochemical efficiency of PSII (F
v/F
m), and superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities decreased less in sense lines than in antisense
lines compared to wild-type (WT) plants. Consistently, the relative electric conductivity,
\textO2 . - {\text{O}}_{2} ^{{. - }} and H2O2 contents in sense lines were lower than those of WT and antisense lines. The antisense lines with low level of unsaturated
fatty acids in PG were extremely susceptible to photoinhibition of PSII and had a significant reduction in the D1 protein
content of PSII reaction center under chilling stress. However, in the presence of streptomycin (SM), the degradation of D1
protein was faster in sense lines than in WT and antisense plants. These results suggested that, under chilling stress conditions,
increasing cis-unsaturated fatty acids in PG through over-expression of LeGPAT can alleviate PSII photoinhibition by accelerating the repair of D1 protein and improving the activities of antioxidant enzymes
in chloroplasts. |
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Keywords: | |
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