Electron microscopy of the K2 killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast |
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| Authors: | AS Vadasz DB Jagganath IS Pretorius AS Gupthar |
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| Institution: | (1) Department of Biochemistry, University of Durban-Westville, Private Bag X54001, Durban, 4000, South Africa;(2) Institute for Wine Biotechnology, University of Stellenbosch, Stellenbosch, 7600, South Africa;(3) Department of Biochemistry, University of Durban-Westville, Private Bag X54001, Durban, 4000, South Africa |
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| Abstract: | A mesophilic wine yeast, Saccharomyces cerevisiae CSIR Y217 K
–
R
– was subjected to the K2 killer effect of Saccharomyces cerevisiae T206 K
+
R
+ in a liquid grape medium. The lethal effect of the K2 mycoviral toxin was confirmed by methylene blue staining. Scanning electron microscopy of cells from challenge experiments revealed rippled cell surfaces, accompanied by cracks and pores, while those unaffected by the toxin, as in the control experiments, showed a smooth surface. Transmission electron microscopy revealed that the toxin damaged the cell wall structure and perturbed cytoplasmic membranes to a limited extent. |
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| Keywords: | electron microscopy killer effect Saccharomyces cerevisiae |
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