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Western blot analysis of the exotoxin components from Bacillus anthracis separated by isoelectric focusing gel electrophoresis
Authors:Little Stephen F
Affiliation:Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702-5011, USA. stephen.little@amedd.army.mil
Abstract:The components of the Bacillus anthracis exotoxins, protective antigen (PA), lethal factor (LF), and edema factor (EF), from 24 isolates were separated by isoelectric focusing gel electrophoresis and detected by Western blot with monoclonal antibodies. Only two isoforms each were observed for PA and EF. Four isoforms were identified for LF. The biological activities of both lethal toxin and edema toxin were measured by using in vitro cell-based assays. This study provides another method of characterizing various isolates of B. anthracis by determining the isoelectric points of the exotoxin components and may be useful in the development of protective vaccines against B. anthracis infection.
Keywords:Bacillus anthracis   Anthrax   Exotoxin   Isoelectric focusing gel electrophoresis
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