Regeneration of an anticarcinogenic herb, Curculigo orchioides (Gaertn.) |
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Authors: | Alice Clara Augustine L D’Souza |
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Institution: | (1) Laboratory of Applied Biology, St. Aloysius College, 575 003 Mangalore, India |
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Abstract: | Summary
Curculigo orchioides is an endangered anticarcinogenic herb. It is available only during the monsoon season, which lasts approximately 4 mo. each
year. In vitro culture of the plant can ensure its availability throughout the year. Leaf explants of Curculigo orchioides cultured on a Murashige and Skoog (MS) medium without cytokinins produced a limited number of plantlets that originated directly
from the cut end of the midrib. 6-Benzyladenine (BA) (0.44–6.66 μM) was needed to produce plantlets from rhizome explants. A higher concentration of BA (2.22–4.44 μM) resulted in nodular callus that when transferred to cytokinin-free medium formed shoots. The shoots were rooted on media
supplemented with either (0.54–5.37 μM) of 1-naphthaleneacetic acid (NAA) (0.57–5.71 μM) of indole-3-acetic acid (IAA), or (0.49–4.90 μM) indole-3-butyric acid (IBA). Plantlets were kept in sterile sand for 3–4 d and then transferred to soil. |
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Keywords: | antineoplastic endangered species Curculigo orchioides regeneration shoot morphogenesis micropropagation |
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