Physical properties of the DNA of bacteriophage SP50 |
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| Authors: | N. Biswal A. K. Kleinschmidt H. C. Spatz T. A. Trautner |
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| Affiliation: | (1) Dept. of Molecular Biology, University of California, Berkeley;(2) Virus Laboratories, University of California, Berkeley;(3) Dept. of Biochemistry, New York University, School of Medicine, New York, New York;(4) Dept. of Biochemistry, Stanford Medical School, Palo Alto, California;(5) Dept. of Molecular Biology and Virus Laboratories, University of California, Berkeley |
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| Abstract: | Summary The following properties of the DNA of B. subtilis phage SP50 were established: Molecular weight (in Daltons) 102×106 (sedimentation velocity) 97×106 (viscosity) 97×106 (contour lengths of electron micrographs) Base Composition (in % GC) 41.7 (chemical analysis) 44 (melting point) 44 (buoyant density) No unusual bases were observed. The complementary strands of the DNA can be separated. The phage DNA has genuine single strand breaks. The number and distribution of such breaks appears to be determined by the host on which phages were grown.This investigation was supported in part by a Public Health Service research grant GM 13,666 from the National Institutes of General Medical Sciences, AI 01267 from the National Institutes of Allergy and Infectious Diseases, AM 04763 from the National Institutes of Arthritis and Metabolic Diseases; cancer research funds from the University of California; and a grant from the Hartford Foundation. |
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