Integrity of lysosomes in the isolated perfused rat liver before and after exposure to dimethylsulphoxide. |
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| Authors: | D Lee R K Holland A K Groufsky |
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| Affiliation: | Department of Surgery, University of Otago Medical School, P.O. Box 913, Dunedin, New Zealand |
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| Abstract: | We have studied the integrity of lysosomes in isolated rat livers perfused for 3, 4, or 6 hr at 35 °C with BSA (40 g/l) in Krebs Ringer bicarbonate buffer. The latency and sedimentability of β-glucuronidase in homogenates of these livers was well maintained even after 6 hr. The latency and sedimentability of acid phosphatase remained at about control levels during the first 4 hr of perfusion but decreased between 4 and 6 hr. These decreases in latency and sedimentability correlated with a decrease in bile production and an increase in the rate of release of GOT into the perfusate and could indicate either intracellular disruption of lysosomes Latencies, Sedimentabilities, and Specific Activities of Acid Phosphatase and β-Glucuronidase in Homogenates of Rat Liver Prepared before or at Various Times after Exposure to 1.4 m Me2SO for 1 hr | Time (hr) | 0 | 3 | 4 | 6 | | Acid phosphatase | | | | | | Latency (%) | 83.2 ± 0.8 | 64.7 ± 5.1 | 62.4 ± 7.9 | 68.9 ± 5.4 | | Sedimentability (%) | 81.7 ± 0.6 | 77.6 ± 3.1 | 81.0 ± 3.4 | 79.4 ± 5.1 | | Specific activity (mIU/mg protein) | 2.8 ± 0.4 | 2.8 ± 0.2 | 2.4 ± 0.4 | 1.6 ± 0.2 | | β-Glucuronidase | | | | | | Latency (%) | 06.8 ± 1.5 | 71.3 ± 4.3 | 74.2 ± 2.5 | 63.3 ± 4.5 | | Sedimentability (%) | 69.5 ± 0.3 | 75.4 ± 3.2 | 75.0 ± 1.1 | 74.6 ± 2.0 | | Specific activity (mIU/mg protein) | 1.8 ± 0.1 | 1.6 ± 0.1 | 1.6 ± 0.2 | 1.6 ± 0.2 |
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