应用AFLP分析梅根系与其根围土壤丛枝菌根真菌DNA多态性差异

应用巢式PCR并进行梅根系与根围土壤丛枝菌根真菌（arbuscular mycorrihizal fungi,AMF）DNA扩增片段长度多态性（amplified fragment length polymorphism,AFLP）的差异比较,研究梅共生AMF的作用机理。结果表明,从18个梅品种的30个根围土壤样品中有28个样品获得纯化的DNA片段,占样品数93.3%,样品平均多态性位点数为6.5个,Nei’s基因多样性为0.3559±0.1382,Shannon信息指数为0.5299±0.1676。与梅根系AMF DNA多态性比较,根围土壤的平均多态性位点数明显较多;且根系AMF的DNA多态性位点绝大多数存在于土壤AMF的DNA多态性位点中,表明根系内AMF是由土壤AMF发育而来;根系与根围土壤AMF DNA的聚类均与梅品种群、品种关联性不强,表明AMF对宿主梅品种或品种群没有特异的共生关系。

DNA polymorphism difference between root system and rhizosphere soil arbuscular mycorrhizal fungi of Prunus mume by AFLP analysis

DNA polymorphism of arbuscular mycorrhizal fungi (AMF) in root system and those in rhizosphere soil of Prunus mume was analyzed through DNA amplification by nested PCR based on AFLP marker. The results show that the purified DNA can be extracted from 28 soil samples, accounting for 93.3% of totally 30 samples, collected from rhizosphere of 18 cultivars, averaging 6.5 loci for each sample. The average genetic identity was 0.3559±0.1382, Shannon information index was 0.5299±0.1676. The number of loci in rhizosphere soil was much more than that of loci in root sample, and most of loci in root sample could be found in soil sample. It was proved that the AMF in root might be developed from soil AMF. The clustered groups of AMF DNA by AFLP marker both from roots and from soils were not connected with cultivar groups or cultivars of P. mume, and indicated that there was no specific symbiotic relationship between AMF and P. mume.