猴头菌子实体小分子提取物的分离与体外抑制幽门螺旋杆菌的分析

本研究以甲硝唑为阳性对照,利用HPD-100大孔树脂和95%乙醇将猴头菌子实体中小分子活性物质进行富集,得到乙醇制备液（MREEs）;然后对乙醇制备液分别使用石油醚（60-90℃）和氯仿进行多次萃取,得到石油醚萃取物（A1、A2）和氯仿萃取物（B）,并进行GC-MS测定;对MREEs和A1、A2、B分别进行药敏试验,同时对5种幽门螺旋杆菌进行最低抑菌浓度测定。结果表明,使用大孔树脂得到的MREEs有较好的抑菌活性,得到的A1、A2和B对幽门螺旋杆菌的抑菌率均高于40.0%,其中石油醚萃取物A1在Helicobacter pylori SSI中的抑菌率甚至高达62.9%;A1、A2和B使用GC-MS测定出的主要成分分别是邻苯二甲酸二丁酯（A1,4.53%和A2,6.93%）和棕榈酸2.87%,但仍有大量成分没有被测出。另外,在最低抑菌浓度试验中,A1相应组分对Helicobacter pylori W₂504、Helicobacter pylori 9和Helicobacter pylori ATCC 43504的最低抑菌浓度都为0.25mg/mL,A2对Helicobacter pylori 78的最低抑菌浓度仅为0.25mg/mL,而B对Helicobacter pylori W₂504、Helicobacter pylori 9的最低抑菌浓度均仅为0.125mg/mL,说明通过石油醚和氯仿萃取得到的猴头菌子实体提取物对幽门螺旋杆菌具有潜在的抑菌作用。

Isolation and antimicrobial activity against Helicobacter pylori in vitro of low molecular weight bioactive components from Hericium erinaceus

MREEs was prepared by enriching the low molecule weight bioactive components extracted from the fruiting bodies of Hericium erinaceus using 95% ethanol in HPD-100 macroporous resin with the metronidazole as positive control. The MREEs were leached by petroleum ether (60-90°C) and chloroform for several times and three extractions were isolated which were encoded as A1, A2 and B and were also detected by GC-MS. The anti-Helicobacter pylori activities of the MREEs, A1, A2 and B were performed through antimicrobial susceptibility test, and the minimum inhibitory concentration (MIC) of five Helicobacter pylori strains was then determined. The results indicated that the MREEs had obvious inhibition effect on the growth of Helicobacter pylori and the inhibition rate of the three extracts (A1, A2, and B) was higher than 40.0%, especially for A1 of which the inhibition rate was as high as 62.9%. The main components of A1, A2, B analyzed by GC-MS were DBP (A1, 4.53% and A2, 6.93%) and palmitic acid (2.87%). In addition, The MIC of A1 against Helicobacter pylori W₂504, Helicobacter pylori 9 and Helicobacter pylori ATCC 43504 was 0.25mg/mL, and MIC of A2 against Helicobacter pylori 78 was 0.25mg/mL. B was the most potent fraction against Helicobacter pylori W₂504 and Helicobacter pylori 9, whose MIC was only 0.125mg/mL. In conclusion, extractions with petroleum ether and chloroform from Hericium erinaceus presented strongly inhibitory activity against Helicobacter pylori.