撕裂蜡孔菌在开放体系中对甲基橙染料的静态脱色研究

为了评价撕裂蜡孔菌处理偶氮染料的应用潜力,用性能稳定的甲基橙染料为材料,采用批次试验在开放性体系中研究了染料初始浓度、菌丝生物量、温度、pH等因素对该菌脱色能力的影响,运用菌丝体反接、染液光谱扫描、菌丝体显微观察等方法探讨了菌丝体脱色的可能机制,利用植物萌发试验进行了染料和脱色后溶液的毒性测试。结果表明,撕裂蜡孔菌在开放的静止体系中能够对甲基橙高效脱色,其最适脱色温度为35℃,最佳脱色pH值在6左右。菌丝对甲基橙的脱色表现在吸附和产酶降解两个方面,脱色过程中染料对菌丝体本身的影响较少。植物毒性分析显示撕裂蜡孔菌脱色48h后的产物对植物的毒性比甲基橙本身更强,若要彻底降解可能需要较长时间。本研究可为染料脱色工艺提供新的菌种。

Decolorization of methyl orange dye by Ceriporia lacerata under statically air-opened condition

In order to evaluate the application potential of Ceriporia lacerata in dealing with the azo dye, methyl orange dye with stable performance was used as material in batch experiments for investigating the effect of initial dye concentration, fungal biomass, temperature and pH on the decolorization of methyl orange dye by C. lacerata. Decolorization mechanism was probed by means of mycelium reverse inoculation, dye solution absorption spectrum and mycelium microexamination. The toxicity test for original dye and decolorizing products using plant seed germination method was conducted. Results showed that C. lacerata effectively decolorized methyl orange dye, and the optimal temperature and pH were 35°C and 6 respectively. The decolorization of dye was caused by both mycelium absorption and enzyme degradation. During the course of decolorization methyl orange dye had little effect on the mycelium. Toxicity test suggested that the products in 48h of decolorization by C. lacerata had more toxicity than original dye. C. lacerata was a useful potentially fungus in the treatment of azo dye.