前体及诱导子和发酵条件对烟曲霉TMS-26产紫杉醇发酵体系优化

紫杉醇是一种广谱的抗癌药物,因其具有独特的抗癌机制、良好的抗癌效果和供不应求的市场等特征而备受关注。紫杉醇具有重大经济效益,但产量受到制约,价格极为昂贵,通过内生真菌发酵法生产紫杉醇能在一定程度上缓解其来源困难的问题。在产紫杉醇内生真菌TMS-26发酵液中添加前体物质和诱导子,并通过对接种量、装液量、初始pH和发酵时间等条件进行优化研究。单因素及正交试验表明在PDB培养基中加入苯丙氨酸20mg/L、苯甲酸钠30mg/L、乙酸钠8g/L、甘氨酸15mg/L、CuSO₄ 0.05mg/L、H₂O₂ 6mmol/L、3,5-二硝基水杨酸15mg/L时能有效提高紫杉醇产量,比优化前增产46.64%,达到446.28µg/L,并且发现最适菌株TMS-26的发酵条件为pH7.5、接种量5%、装液量120mL/250mL、发酵时间为10d。

Optimization of Aspergillus fumigatus TMS-26 taxol production fermentation system by precursors,elicitors and fermentation conditions

Taxol, a well-known anti-cancer medicine, is popularly studied due to its unique anti-cancer mechanism, excellent anti-cancer effects, and its tight market. Although taxol has great economic benefits, its price is quite high because of its restricted production. Producing taxol through endophytic fungus fermentation method can solve this difficult situation. In this paper, we reported the effects of the addition of precursors and elicitors to taxol-producing endophytic fungus Aspergillus fumigatus TMS-26 broth, and the optimization of inoculum density, loading volume, initial pH value and fermentation duration for taxol production. Results showed that the maximum taxol yield could reach 446.28µg/L, with 46.61% increase as compared with that by traditional method. Maximum yield was achieved when concentrations of phenylalanine, sodium benzoate, sodium acetate, glycine, CuSO₄, H₂O₂ and 3,5-dinitrosalicylic acid were 20mg/L, 30mg/L, 8g/L, 15mg/L, 0.05mg/L, 6mmol/L and 15mg/L respectively. Optimal fermentation condition for the strains was obtained under pH7.5, inoculation quantity of 5%, liquid volume of 120mL/250mL and fermentation duration of 10 days.