Gene expression microarray analysis of early oxygen-induced retinopathy in the rat |
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| Authors: | Melinda Tea Rhys Fogarty Helen M Brereton Michael Z Michael Mark B Van der Hoek Anna Tsykin Douglas J Coster Keryn A Williams |
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| Institution: | 1Department of Ophthalmology, Flinders University of South Australia, Adelaide, Australia ;2Department of Gastroenterology and Hepatology, Flinders University of South Australia, Adelaide, Australia ;3Adelaide Microarray Centre, University of Adelaide & Hanson Institute, Adelaide, Australia ;4Department of Ophthalmology, Flinders Medical Centre, Bedford Park, 5042 SA Australia |
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| Abstract: | Different inbred strains of rat differ in their susceptibility to oxygen-induced retinopathy (OIR), an animal model of human retinopathy of prematurity. We examined gene expression in Sprague–Dawley (susceptible) and Fischer 344 (resistant) neonatal rats after 3 days exposure to cyclic hyperoxia or room air, using Affymetrix rat Genearrays. False discovery rate analysis was used to identify differentially regulated genes. Such genes were then ranked by fold change and submitted to the online database, DAVID. The Sprague–Dawley list returned the term “response to hypoxia,” absent from the Fischer 344 output. Manual analysis indicated that many genes known to be upregulated by hypoxia-inducible factor-1α were downregulated by cyclic hyperoxia. Quantitative real-time RT-PCR analysis of Egln3, Bnip3, Slc16a3, and Hk2 confirmed the microarray results. We conclude that combined methodologies are required for adequate dissection of the pathophysiology of strain susceptibility to OIR in the rat. |
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| Keywords: | Cyclic hyperoxia Gene expression Oxygen-induced retinopathy Affymetrix microarray Inbred rat |
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