Purification and viability determinations of plant protoplasts |
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| Authors: | P J Larkin |
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| Institution: | (1) School of Botany, University of New South Wales, 2033 Kensington, N.S.W., Australia |
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| Abstract: | Summary A method is described for purifying plant protoplasts from cellular and subcellular debris. The procedure utilizes a density buffer containing 9.6% sodium metrizoate and 5.6% Ficoll. The use of fluorescein diacetate for assessing the viability of plant protoplasts is also reported.Abbreviation FDA
fluorescein diacetate |
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